CRG/UPF Proteomics Unit

The Proteomics Unit is a joint effort of the Universitat Pompeu Fabra and the Centre of Genomic Regulation to create an innovative research infrastructure with a high-level of excellence and sustainability to support scientists in the biomedical field. The activities of the Proteomics Unit are focused onto the provision of high-quality proteomics services with strong added-value to the research community by i) offering state-of-the-art methods, advise and expertise to support basic and translational researchers; ii) developing new methods and techniques that keep the unit up to date and at the forefront of the proteomics field; and iii) training the community and actively disseminating proteomics science and methods.

The Proteomics Unit is part of the “Plataforma de Recursos Biomoleculares y Bioinformáticos (PRB3 ProteoRed, Instituto de Salud Carlos III)” a national consortium of proteomics units that grants access to state-of-the-art proteomics technology to the national health system.

In 2018, the Proteomics Unit has successfully co-organized the 10th MaxQuant Summer School in Barcelona together with the MaxPlanck Institute of Biochemistry, and the B-Debate on Clinical Proteomics together with VHIO, Parc Cientific de Barcelona, and IDIBELL. The Proteomics Unit has also organized the “EMBO Practical Course on Targeted Proteomics: Experimental Design and Data Analysis”.

• Study labile protein post-translational modifications.
• Characterization of protein interactions in cancer and atherosclerosis.
• Biomarker discovery and validation in cancer and cardiac pathologies.
• Development of targeted proteomics methods.
• Quality assessment of mass spectrometry-based proteomics experiments.

• Excellence Project-Spanish Ministry of Economy and Competitiveness: “Caracterización por espectrometría de masas de las modificaciones post-traduccionales de proteínas mono- y poli(ADP-Ribosa)”. CTQ2016-80364-P
• Retos Project- Spanish Ministry of Economy and Competitiveness: “Desarrollo de una terapia innovadora para el tratamiento de la aterosclerosis mediante la inhibición de la acumulación vascular de colesterol”. RTC-2016-5078-1
• Research Groups Activities Support Grant (SGR). “Proteomics of Complex Systems” 2017 SGR 595

Chiva C, Olivella R, Borràs E, Espadas G, Pastor O, Solé A, Sabidó E.
“QCloud: A cloud-based quality control system for mass spectrometry-based proteomics laboratories.”
PLoS One, 13(1):e0189209, 2018. doi: 10.1371/journal.pone.0189209.

Borràs E, Sabidó E.
“DIA+: A Data-Independent Acquisition Method Combining Multiple Precursor Charges to Improve Peptide Signal.”
Anal Chem, 90(21):12339-12341, 2018. doi: 10.1021/acs.analchem.8b03418.

Belužić L, Grbeša I, Belužić R, Park JH, Kong HK, Kopjar N, Espadas G, Sabidó E, Lepur A, Rokić F, Jerić I, Brkljačić L, Vugrek O.
“Knock-down of AHCY and depletion of adenosine induces DNA damage and cell cycle arrest.”
Sci Rep, 8(1):14012, 2018. doi: 10.1038/s41598-018-32356-8.

Maitre L, de Bont J, Casas M, Robinson O, Aasvang GM, Agier L, Andrušaitytė S, Ballester F, Basagaña X, Borràs E, Brochot C, Bustamante M, Carracedo A, de Castro M, Dedele A, Donaire-Gonzalez D, Estivill X, Evandt J, Fossati S, Giorgis-Allemand L, R Gonzalez J, Granum B, Grazuleviciene R, Bjerve Gützkow K, Småstuen Haug L, Hernandez-Ferrer C, Heude B, Ibarluzea J, Julvez J, Karachaliou M, Keun HC, Hjertager Krog N, Lau CE, Leventakou V, Lyon-Caen S, Manzano C, Mason D, McEachan R, Meltzer HM, Petraviciene I, Quentin J, Roumeliotaki T, Sabido E, Saulnier PJ, Siskos AP, Siroux V, Sunyer J, Tamayo I, Urquiza J, Vafeiadi M, van Gent D, Vives-Usano M, Waiblinger D, Warembourg C, Chatzi L, Coen M, van den Hazel P, Nieuwenhuijsen MJ, Slama R, Thomsen C, Wright J, Vrijheid M.
“Human Early Life Exposome (HELIX) study: a European population-based exposome cohort.”
BMJ Open, 8(9):e021311, 2018. doi: 10.1136/bmjopen-2017-021311.

Rosas-Salvans M, Cavazza T, Espadas G, Sabido E, Vernos I.
“Proteomic Profiling of Microtubule Self-organization in M-phase.”
Mol Cell Proteomics, 17(10):1991-2004, 2018. doi: 10.1074/mcp.RA118.000745.

Ferrer I, García MA, González IL, Lucena DD, Villalonga AR, Tech MC, Llorens F, Garcia-Esparcia P, Martinez-Maldonado A, Mendez MF, Escribano BT, Bech-Serra JJ, Sabido E, de la Torre Gómez C, Del Rio JA.
“Aging-related tau astrogliopathy (ARTAG): not only tau phosphorylation in astrocytes.”
Brain Pathol, 28(6):965-985, 2018. doi: 10.1111/bpa.12593.

A European patent application “Improvements in Mass Spectrometry” was filed on 1 June 2018 through the Spanish Patent Office, with European Patent Application No. 18382384.8