Biomolecular Screening and Protein Technologies Unit

The Biomolecular Screening & Protein Technologies Unit (BMS&PT) provides researchers at CRG/PRBB, external institutions and private companies with a state-of-the-art technology platforms and broad knowledge and expertise to perform the following services:

1. Full-service cloning technologies for several applications including CRISPR-Cas9 system
2. Protein Expression in bacteria, baculovirus- insect cell and mammalian cell systems
3. Providing rapid and robust solutions for purification of high quality protein using different liquid handling chromatography systems (1x Akta Avant, 2x AKTAxpress, 1x AktaFPLC)
4. Measurement and analysis of protein-protein, protein-nucleic acids and drug-protein interactions. (Biacore T100, ITC, Nanolith NT115)
5. Determination of the native molar mass, oligomeric state and protein-protein complex formation using the size-exclusion multi Angle light scattering (SEC-MALS) instrument
6. DNA extraction and isolation from environmental samples in the context of Metagenomic projects
7. Automation for screening assays (Tecan evo200 and Sciclone robotic platforms)

• Genome editing reagents for CRISPR-Cas9 applications
• Biomolecular interaction assay development
• Metagenomics DNA Extraction of environmental samples

The unit participated in the Courses@CRG series with the workshop entitled “Tissue Engineering Course: From Stem Cells to Organoids on the session CRISPR-Cas9 design tools.” All members of the staff are frequently participating as speakers and instructors in master courses from the CRG and UPF.

Willis JR, González-Torres P, Pittis AA, Bejarano LA, Cozzuto L, Andreu-Somavilla N, Alloza-Trabado M, Valentín A, Ksiezopolska E, Company C, Onywera H, Montfort M, Hermoso A, Iraola-Guzmán S, Saus E, Labeeuw A, Carolis C, Hecht J, Ponomarenko J, Gabaldón T.
“Citizen science charts two major “stomatotypes” in the oral microbiome of adolescents and reveals links with habits and drinking water composition.”
Microbiome, 6(1):218, 2018.

Sao Emani C, Williams MJ, Van Helden PD, Taylor MJC, Carolis C, Wiid IJ, Baker B.
“Generation and characterization of thiol-deficient Mycobacterium tuberculosis mutants.”
Sci Data, 5:180184, 2018.

Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, Beltran-Sastre V, Serrano L, Maurer SP.
“rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.”
Nat Commun, 9(1):3747, 2018.

Sao Emani C, Williams MJ, Wiid IJ, Baker B, Carolis C.
“Compounds with Potential Activity against Mycobacterium tuberculosis.”
Antimicrob Agents Chemother, 62(4), 2018.